Services

Custom Antibody Development/Production/Generation Services

WE provide a full range of custom antibody development/production services or the life science and biotech industry.

 

Custom Polyclonal Antibody Development/Production Service in Rabbit (2 New Zealand white rabbits)

Salient Features of Custom Polyclonal Antibody Development/Production Service

  • 12 Weeks protocol to produce antibody in 2 New Zealand White Rabbits (From confirmed Pure line Parental stock).
  • This will include 7 immunizations and 3 Production bleeds per animal.
  • Pre-immune sera of 5-10 ml per animal and a total of 30-40 ml of crude immune sera per rabbit.
  • *FREE ELISA Titer at first bleed against free immunogen.

Customer will be notified of immunization schedule and tentative date of production/Progress of the Project etc.

Immunogen required for custom polyclonal antibody development/production 

  1. For Protein (Provided by customer)-3 mg, Concentration: minimum 0.5 mg/ml
  2. For peptide (Min ~5-7 mg)

IMMUNIZATION AND BLEEDING SCHEDULE

WEEKS

PROCEDURE

BLEED/RABBIT

Week 1 (Pre-bleed)
Primary Immunization

Pre-Immune Sera and Primary Immunization

~5 ml*

Week 2 (Immunize)

2nd Immunization

 

Week 3 (Immunize)

3rd Immunization

 

Week 4 (Immunize)

4th Immunization

 

Week 5 (Immunize)

5th Immunization

 

Week 6 (Bleed-1)

1st production Bleed

~10 ml*

Week 7 (Immunize)

6th Immunization

 

Week 8 (Bleed-2)

2nd production Bleed

~10 ml*

Week 9 (Immunize)

7th Immunization

 

Week 10 (Bleed-3)

3rd production Bleed

~10 ml*

 

Extention of project per month (2 bleeds/Rabbit)

Bleed-1

~10 ml*

Bleed-2

~10 ml*

 

Terminal bleed
(1 bleed per Rabbit)

Bleed-1

~20-25 ml*

 

* In our custom polyclonal development/production service we usually provide the above mentioned sera from one rabbit/project but it may vary depending upon the health status of the animal.

The above schedule of custom polyclonal antibody development/production is approximate. We can also modify it according to the customer’s need.

Additional 4 weeks is required if the custom polyclonal antibody development/production service requires peptide synthesis, mass spec, KLH conjugation. For custom polyclonal antibody development service in which the immunogen is supplied can be started immediately. 

Purification:


Protein A : 15-20 mg of purified Antibody
Peptide affinity : 2-3 mg of purified Antibody

 

 

Additional Services

  • Custom peptide synthesis
  • Recombinant protein production for custom polyclonal antibody development/production in India
  • Custom polyclonal antibody development service against Phospho/Acetylated protein in India
  • Custom polyclonal antibody development service in Mice/Rat in India
  • Validation of custom polyclonal antibody for FACS, IP, IHC and IF
  • Custom monoclonal antibody development service in India

Terms and Conditions:
 

If the immunogen is peptide then it must contain a cysteine for conjugation.

  1. If the immunogen is already conjugated or it’s in gel, kindly provide us ~1 mg of free peptide/purified protein for ELISA.
  2. Recombinant protein and purified protein must be shipped in dry ice.
  3. Kindly provide antigen in soluble form.
  4. For peptide affinity purification customer should provide additional 6 mg peptide.
  5. Biostring Inc. guarantees ELISA positive sera for the immunogen synthesized.
  6. The titre of the antisera depends on the quality of the antigen.